I work remotely from home and my colleagues know me via phone and email...plus one company-wide meeting a year. I know virtually nothing about most of my co-workers and they know virtually nothing about me.
As far as my closest work buddies (like my work partner, who describes himself as a fruit)....yes, I'm out. My work partner's statement when he saw a pic of Scoote... "ooooohhhh she's all cute and butch and stuff."
My company includes full same-sex partner benefits and includes "sexual and gender orientation" in their non-discrimination policy. I only ever heard of one person giving gay colleagues a difficult time. He was fired.